Patrice G. Bouyer

Patrice G. Bouyer

Associate Professor of Biology patrice.bouyer@valpo.edu 219.464.5487 Neils Science Center, 301

Education

  • Ph.D. – René Descarte University, Paris (1998)
  • DEA (Masters) – René Descarte University, Paris (1994)
  • Maîtrise de Physiologie mention pharmacologie – University of Poitiers, France (1993)
  • Licence de Biologie Cellulaire mention Physiologie – University of Poitiers, France (1992)
  • Diplôme d’études universitaires générales (DEUG) – University of Poitiers, France (1990)

Research Interests

The main research focus of the lab is to understand the regulation of the host defense mechanism: gut clearance (fluid secretion). We are studying the fluid secretion mechanism in normal conditions and during its subversion by the fungus C. albicans. The secreted fluid flushes away microorganisms to prevent them from attaching to the cell surfaces and subsequently invading the host. The cellular fluid secretion machinery is complex and requires many proteins to work together such as the basolateral Na+-K+-2Cl– cotransporter 1 (NKCC1) and the apical chloride channels. The regulation of fluid secretion by NKCC1 is still poorly understood. During host invasion, C. albicans shifts its morphology from yeast to filament, but the gut environmental factors responsible for that shift are still unclear. Furthermore, the cellular mechanisms underlying the epithelium invasion by C. albicans remain to be clarified. The lab currently has three projects:

  1. Regulation of fluid secretion by the basolateral Na+-K+-2Cl– cotransporter
  2. Gut environmental factors responsible for the morphology shift of C. albicans
  3. The cellular and molecular mechanisms subverted by C. albicans during host invasion

The lab uses cell culture models, biochemical and fluorescent/bright field microscopy techniques to delineate the mechanisms in the three projects. For more on the research in my lab visit: https://sites.google.com/valpo.edu/patrice-bouyer-research-lab/home

Contributions

  • Bouyer PG, Tang X, Weber CR, Shen L, Turner JR & Matthews JB. 2013. Capsaicin induces NKCC1 internalization and inhibits chloride secretion in colonic epithelial cells independently of TRPV1.  Am J Physiol Gastrointest & Liver Physiol 304: G142-156.
  • Chlenski A, Guerrero LJ, Salwen HR, Yang Q, Tian Y, Morales La Madrid A, Mirzoeva S, Bouyer PG, Xu D, Walker M & Cohn SL. 2011. Secreted Protein Acidic and Rich in Cysteine Is a Matrix Scavenger Chaperone. PLoS One, 6: e23880.
  • Tang J, Bouyer P, Mykoniatis A, Buschmann M, Matlin, KS & Matthews JB. 2010. Activated PKCδ and PKCϵ inhibit epithelial chloride secretion response to cAMP via inducing internalization of the Na+-K+-2Cl– cotransporter NKCC1. J Biol Chem, 285: 34072-34085.
  • Mykoniatis A, Shen L, Fedor-Chaiken, M, Tang J, Tang X, Worrell RT, Delpire E, Turner JR, Matlin KS, Bouyer P*, & Matthews JB. 2010. Phorbol 12-myristate 13-acetate-induced endocytosis of the Na-K-2Cl cotransporter in MDCK cells is associated with a clathrin-dependent pathway. Am J Physiol Cell Physiol, 298: C85-97 (* senior corresponding author).